well rabbit novus biologicals nbp1 48336 ldh a antibody blocking peptide elisa detection n a n a novus biologicals nbp1 48336pep ldh b elisa detection  (Novus Biologicals)

Journal: International Journal of Molecular Sciences

Article Title: GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia

doi: 10.3390/ijms23169375

Figure Lengend Snippet: List of antibodies and antibody-blocking peptides used in the study.

Article Snippet: LDH-A antibody blocking peptide , ELISA detection , N/A , N/A , Novus Biologicals , NBP2-38131PEP , N/A.

Techniques: Enzyme-linked Immunosorbent Assay, Blocking Assay, Peptide ELISA, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia

doi: 10.3390/ijms23169375

Figure Lengend Snippet: miR-210 mitigates the hypoxia-induced caspase-3 activation-mediated cell death through the inhibition of GSK3β kinase activity. ( A ) Quantitative ELISA determining the LDH release in the conditioned media as a surrogate marker of cell death. Data from the LDH ELISA are expressed as experimental blank-corrected absorbances (O.D) measured at λ 450 (450 nm). ( B ) Qualitative Western blot depicting the processing of the inert procaspase-3 (p32) into the active cleaved-caspase-3 (p17 fragment) as a surrogate measure of caspase-3 activation status ( C ) Quantitative ELISA determining the levels of the active cleaved-caspase-3 (p17 fragment), expressed as experimental blank-corrected absorbances (O.D) measured at λ 570 (570 nm). ( D ) Qualitative Western blot depicting the processing of the caspase-3 substrate PARP (p116) into the cleaved -PARP (p89 fragment) as a surrogate measure of caspase-3 activation status. ( E ) Quantitative ELISA determining the levels of the cleaved -PARP (p89 fragment), expressed as experimental blank-corrected absorbances (O.D) measured at λ 570 (570 nm). ( F ) Quantitative caspase-3 activity assay in native non-denatured lysates, expressed as experimental blank-corrected absorbances (O.D) measured at λ 405 (405 nm). miR-210 expression levels in the respective cell lysates were determined by the miR-210 hybridization immunoassay (as described in ) and are reported in . The validation of the ectopic expression of the HA-tagged ca -GSK3β-S9A mutant in the pertinent experimental groups was performed by Western blot analysis ( B ) as well as ELISA immunoassay and is reported in . GSK3β kinase activity (as described in ) was measured in all experimental groups to corroborate and validate the translative effects of the ectopic expression of the ca -GSK3β-S9A mutant (reported in ). All data are expressed as mean ± S.D from three technical replicates for each of the four biological replicates belonging to each experimental group ( n = 4). * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001; ns: not significant ( p > 0.05). OE: miR-210 overexpression; O.D: optical density; S.D: standard deviation.

Article Snippet: LDH-A antibody blocking peptide , ELISA detection , N/A , N/A , Novus Biologicals , NBP1-48336PEP , N/A.

Techniques: Activation Assay, Inhibition, Activity Assay, Enzyme-linked Immunosorbent Assay, Marker, Western Blot, Caspase-3 Activity Assay, Expressing, Hybridization, Biomarker Discovery, Mutagenesis, Over Expression, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia

doi: 10.3390/ijms23169375

Figure Lengend Snippet: List of antibodies and antibody-blocking peptides used in the study.

Article Snippet: LDH-A antibody blocking peptide , ELISA detection , N/A , N/A , Novus Biologicals , NBP1-48336PEP , N/A.

Techniques: Enzyme-linked Immunosorbent Assay, Blocking Assay, Peptide ELISA, Activity Assay